Resources Archive

Nano eNabler Product Sheet

Benchtop Molecular Printer

  • Sensor Functionalization
  • Lab-on-a-Chip
  • Cell Patterning

You don’t just have one application idea, you have several – and you need a versatile surface patterning instrument that will allow you to explore all of the possibilities. The Nano eNabler can help turn those concepts into reality, so you can move ahead to the next big challenge.

Continue Reading

Custom Surface Patterning Service

Problem:  You need a custom pattern printed onto a surface for a grant or proof-of-concept study, and you do not have access to a Nano eNabler or Cyto eNabler in your lab.

Solution:  Let BioForce help design, optimize, and print those patterned surfaces for you.

Continue Reading

Application Note 109 – Use of Patterned Sindex™ Chips for Rapid Ultramicroarray Localization

We demonstrate the utility of the proprietary Sindex™ chip for creation and relocalization of ultraminiaturized bioarrays. Due to the extreme precision and miniaturization made possible by the Nano eNabler™ system, a “map” is required to allow the user to find locations of ultraminiaturized patterns on their test substrates. The Sindex™ chip meets this need with an addressable alphanumeric grid etched directly onto the silicon surface.

Continue Reading

Application Note 110 – ViriChip™ platform for Ultraminiaturized Direct Sensing Virus Detection

In this application note, we demonstrate a novel label free method for direct virus sensing. The Nano eNabler™ system is used to create ultraminiaturized antibody arrays with spot sizes (10 to 15 µm) in discrete locations. These spots occupy 1/100th to 1/1000th the surface area of a conventional microarray (D=100 µm) spot [1]. The ultraminiaturized spatial scale made possible by the Nano eNabler™ system creates an opportunity for direct readout by surface profiling using the principles of atomic force microscopy (AFM) along with more conventional methods. Viruses remain bioactive and amenable to culture and PCR.

Continue Reading

Application Note 104 – Direct Deposition of QDs in Discrete Ultramicro Domains

Quantum Dots are proving to have a broad range of utility in the biosciences and elsewhere. In this application note we demonstrate that ultramicro-scale placement of QDs onto surfaces is possible with the Nano eNabler™ system. This suggests that it is possible to use the Nano eNabler™ system  to build QD-based devices and position QDs on a variety of biological samples, including living cells.

Continue Reading

Application Note 105 – Combing Large DNAs on Surfaces using the Nano eNabler System

The ability to place large DNAs at specific locations with ultramicroscale precision is of paramount importance in the development of forward-looking technologies. This demonstration illustrates that the Nano eNabler™ system can be used to deposit large DNA molecules and also align them in a roughly parallel orientation. This ability could serve as the basis for creating DNA chips with many different DNA fragments aligned side by side for rapid DNA mapping and sequencing.

Continue Reading

Application Note 113 – Deposition of Oligonucleotides using the Nano eNabler™ System

Gene expression analysis using DNA microarrays [1] has become a standard tool for profiling disease states and monitoring efficacy of therapeutic agents. Typical spotted microarrays have relatively large oligonucleotide spots measuring 100-200 um, so a reduction in spot diameter presents an opportunity to reduce sample volume requirements, improve binding kinetics, and possibly even eliminate the need for sample amplification. We investigated the feasibility of printing oligonucleotides onto a functionalized surface using the Nano eNabler™ system [2,3].

Continue Reading

Application Note 107 – Enabling Ultraminiaturized Biosensors with the Nano eNabler™ System

In the last decade a lot of progress has been made in designing and constructing microfabricated (and nanofabricated) biosensing devices. A major hurdle yet to be overcome is the specific functionalization of these individual micro- and nanodevices with affinity capture molecules. In this application note, we describe our solution to this problem and how it will enable a new generation of biosensing devices for medical, environmental and biodefense applications.

Continue Reading

Application Note 112 – Ultramicroscale Patterning of UV Curable Adhesive

The Nano eNabler™ system is used to deposit femtoliter volumes of a UV curable adhesive onto a silicon Sindex™ chip. This capability has applications in the MEMS and semiconductor industries for the bonding of intricate microfabricated parts and surface mount microelectronic components, as well as underfilling of dies. UV curable adhesives may also be mixed with nanomaterials to create patterned microstructures with unique properties.

Continue Reading

Application Note 102 – Low Level Detection of Protein Biomarkers from Cancer Cells

In this application note, we demonstrate a novel method for detecting Prostate Serum Antigen (PSA) from reduced sample volumes. The Nano eNabler™ system is used to create ultraminiaturized antibody arrays with spot sizes from 5-8 µm in diameter occupying 1/100th to 1/1000th the surface area of a conventional microarray (D=100 µm) spot [1]. This PSA level of sensitivity is several orders of magnitude lower than that required for biomedically relevant screening. Furthermore, it requires < 1 l of sample, opening the door to minimally invasive cancer biomarker screening and ultimately single cell diagnostics. Serum PSA and IL-6 (interleukin 6) levels are part of the early detection and prognostic tests for prostate cancer, and provide a good model system for evaluating this detection methodology.

Continue Reading