Application Note 113 – Deposition of Oligonucleotides using the Nano eNabler™ System

Gene expression analysis using DNA microarrays [1] has become a standard tool for profiling disease states and monitoring efficacy of therapeutic agents. Typical spotted microarrays have relatively large oligonucleotide spots measuring 100-200 um, so a reduction in spot diameter presents an opportunity to reduce sample volume requirements, improve binding kinetics, and possibly even eliminate the need for sample amplification. We investigated the feasibility of printing oligonucleotides onto a functionalized surface using the Nano eNabler™ system [2,3].

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