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Application Note 102 – Low Level Detection of Protein Biomarkers from Cancer Cells

In this application note, we demonstrate a novel method for detecting Prostate Serum Antigen (PSA) from reduced sample volumes. The Nano eNabler™ system is used to create ultraminiaturized antibody arrays with spot sizes from 5-8 µm in diameter occupying 1/100th to 1/1000th the surface area of a conventional microarray (D=100 µm) spot [1]. This PSA level of sensitivity is several orders of magnitude lower than that required for biomedically relevant screening. Furthermore, it requires < 1 l of sample, opening the door to minimally invasive cancer biomarker screening and ultimately single cell diagnostics. Serum PSA and IL-6 (interleukin 6) levels are part of the early detection and prognostic tests for prostate cancer, and provide a good model system for evaluating this detection methodology.

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