In this application note the Nano eNabler™ system is used to print proteins onto various surface types including hydrogel, nitrocellulose, and chemically treated surfaces such as silanes and self-assembling monolayers (SAMs). This demonstrates the utility of these surfaces as printing substrates for a variety of potential applications, some of which may require a flat silane or […]
Resource Types Archives: Application Notes
The potential of printed arrays containing multiple proteins in complex or even interlaced patterns is limitless for the arraying and subsequent monitoring individual, indexed cells. In this application note, the flexible, multiplexed printing of utilizing the NanoeNabler™ is demonstrated.
Aldehyde-modified 3-aminopropyltriethoxysilane (APTES) can be employed to covalently bind amines; or, rather, the exposed amino groups of a polypeptide printed with the Nano eNabler™ to borosilicate glass.
Although using the laser for printing arrays controls the “contact force” of the cantilever on the substrate and this is useful for highly quantitative applications; however, applications, such as printing arrays for cell binding, do not require a consistent contact force. Because consistent contact force requires the laser and subsequent associated processing time, printing in […]
The standard SPT holder that comes with the Nano eNabler system™ holds the SPT at a 12° angle with respect to the surface. Smaller spots with better morphology and closer spacing can be achieved by using optional, alternate angled SPT holders that hold the cantilever at 24°, 36°, or 48°. Liquids may also be dispensed […]
Epoxysilane SAM (self-assembling monolayers) surfaces on glass or silicon are effective as a substrate for creating protein patterns. Epoxysilanes (glycidoxypropyltrimethoxysilane) can be employed to covalently bind primary amines; and, exposed amino groups of polypeptides printed with the Nano eNabler™ to borosilicate glass.
William Montes and Thomas C. Marsh, University of St. Thomas, Dept. of Chemistry The Nano eNabler (NeN) is a versatile micro/nano-scale printing tool for creating arrays of materials with high precision and accuracy. An open channel microfluidic device is used to deliver very small volumes of solution to a surface. The majority of current applications […]
Indexing living, eukaryotic cells to discrete, addressable loci on surfaces is critical to furthering studies of development, differentiation, response to stimuli, cell-cell communication, regeneration, and in forms of bioelectronics, suturing and tissue replacement. This can be accomplished by employing the Nano eNabler™ to generate arrays of extra-cellular matrix proteins (ECM’s) on APTES-aldehyde, epoxysilane, or polystyrene […]