Resource Types Archives: Application Notes

We demonstrate the utility of the proprietary Sindex™ chip for creation and relocalization of ultraminiaturized bioarrays. Due to the extreme precision and miniaturization made possible by the Nano eNabler™ system, a “map” is required to allow the user to find locations of ultraminiaturized patterns on their test substrates. The Sindex™ chip meets this need with […]

In this application note, we demonstrate a novel label free method for direct virus sensing. The Nano eNabler™ system is used to create ultraminiaturized antibody arrays with spot sizes (10 to 15 µm) in discrete locations. These spots occupy 1/100th to 1/1000th the surface area of a conventional microarray (D=100 µm) spot [1]. The ultraminiaturized […]

Quantum Dots are proving to have a broad range of utility in the biosciences and elsewhere. In this application note we demonstrate that ultramicro-scale placement of QDs onto surfaces is possible with the Nano eNabler™ system. This suggests that it is possible to use the Nano eNabler™ system  to build QD-based devices and position QDs […]

The ability to place large DNAs at specific locations with ultramicroscale precision is of paramount importance in the development of forward-looking technologies. This demonstration illustrates that the Nano eNabler™ system can be used to deposit large DNA molecules and also align them in a roughly parallel orientation. This ability could serve as the basis for creating […]

Gene expression analysis using DNA microarrays [1] has become a standard tool for profiling disease states and monitoring efficacy of therapeutic agents. Typical spotted microarrays have relatively large oligonucleotide spots measuring 100-200 um, so a reduction in spot diameter presents an opportunity to reduce sample volume requirements, improve binding kinetics, and possibly even eliminate the […]

In the last decade a lot of progress has been made in designing and constructing microfabricated (and nanofabricated) biosensing devices. A major hurdle yet to be overcome is the specific functionalization of these individual micro- and nanodevices with affinity capture molecules. In this application note, we describe our solution to this problem and how it […]

The Nano eNabler™ system is used to deposit femtoliter volumes of a UV curable adhesive onto a silicon Sindex™ chip. This capability has applications in the MEMS and semiconductor industries for the bonding of intricate microfabricated parts and surface mount microelectronic components, as well as underfilling of dies. UV curable adhesives may also be mixed with […]

In this application note, we demonstrate a novel method for detecting Prostate Serum Antigen (PSA) from reduced sample volumes. The Nano eNabler™ system is used to create ultraminiaturized antibody arrays with spot sizes from 5-8 µm in diameter occupying 1/100th to 1/1000th the surface area of a conventional microarray (D=100 µm) spot [1]. This PSA […]

This application describes some of the salient features of the ensemble of proprietary SPT™ print cartridges we have developed for a variety of purposes. SPT™ print cartridges are compatible with biomaterials and non-biological materials, allowing them to place, position and pattern a broad range of materials on surfaces.

The Nano eNabler™ system is used to directly deliver attoliter to picoliter quantities of an etchant to a gold surface. The result is the production of ultraminiaturized holes in the gold surface, revealing the silicon substrate beneath. This process can be used to create microwells, ultramicrostructures, localized catalytic or inert domains, and ultramicroscale structural elements for […]